Study the Pathological Effects of Crude Extract of Portulaca Oleracea L. in the Treatment of Transplanted Mammary Tumor in Female Albino Mice

This study was designed to evaluate the therapeutic effect of 70% ethanolic crude extract of Portulaca oleracea L on mice mammary adenocarcinoma (in vivo). In vivo, the acute toxicity of 70 % ethanolic extract of the plant on normal mice has been studied. No toxic effect was noticed on normal mice even at 9500 mg /kg B.W S/C injection. Therapeutic effect of ethanolic extract of Portulaca oleracea was studied on tumorbearing female mice after S/C administration at dose of 200 mg/kg B.W for 30days (group II), compared with tumorbearing female mice (group I) were injected with D.W only and served as control (+). While healthy female mice (group III) injected with D.W only and served as a control (-). The results showed significant reduction in tumor volume, relative tumor volume and inhibition of tumor growth rate in treated mice (group II) compared with tumor-bearing female mice of non-treated group (group I), which showed increased tumor volume. Gross lesion of tumor mass (group II) showed a small size tumor mass and localized S/C region. Histologically, showed extensive tumor cell necrosis in the center, surrounded by a thick band of granulation tissue which is infiltrated with mononuclear cells (lymphocyte and macrophage). Compared with gross lesion of tumor mass (group I) which revealed that large, irregular tumor mass, with highly vascularzation. Histologically, there was extensive tumor growth which consist of aciner like structure, involving C.T stroma of mammary gland, the tumor cells are hyperchromatic, pleomorphic, giant tumor cell formation and certain section showed mitotic figure, some tumor growth showed extensive coagulation necrosis in the center, with area of calcification.